Lawrence Palmer, Ph.D.

Professor of Physiology and Biophysics

  • Thomas H. Meikle Jr., Professor of Medical Education

212-746-6355

1300 York Avenue, Room C-501 C
New York, NY 10065


Techniques

Research Areas


Research Summary:

The Palmer lab studies the mechanisms of epithelial ion transport and its regulation by hormones and other factors.  Part of this research is at the molecular level.  Here we identify ion channel proteins that mediate the movement of Na+and K+into or out of epithelial cells, and explore the aspects of those proteins that confer the properties of ion conduction and selectivity for the cognate ion.  Epithelial Na+channels (ENaCs) for example, are exquisitely selective for Na+over K+but the basis for that selectivity is not well understood.

 

 

 

Experimental techniques include electrophysiology, both whole-cell and single-channel, heterologous expression systems and site-directed mutagenesis. A second line of investigation explores the processes that regulate these channels in cells, organs and whole animals. Here a fundamental question is how the kidneys adapt to changes in dietary intake to maintain constant concentrations and amounts of electrolytes in extracellular fluids. Here we look at changes in channel number, location and function in kidney cells under varying physiological conditions. Tools include electrophysiology, Western blotting and RNA sequencing, together with genetically modified animal models. Finally, we are beginning to explore interactions between different segments of the nephron that express different ion transporters in the maintenance of electrolyte homeostasis.

 

Recent Publications:

  1. Li, J, Xu, S, Yang, L, Yang, J, Wang, CJ, Weinstein, AM et al.. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. Am. J. Physiol. Renal Physiol. 2019;317 (4):F967-F977. doi: 10.1152/ajprenal.00125.2019. PubMed PMID:31390232 .
  2. Andersen, OS, Nairn, AC, Palmer, LG, Shapley, RM. In Memoriam: David C. Gadsby, PhD. J. Gen. Physiol. 2019;151 (8):967-969. doi: 10.1085/jgp.201912400. PubMed PMID:31311825 PubMed Central PMC6683670.
  3. Yang, L, Palmer, LG. Determinants of selective ion permeation in the epithelial Na+ channel. J. Gen. Physiol. 2018;150 (10):1397-1407. doi: 10.1085/jgp.201812164. PubMed PMID:30135076 PubMed Central PMC6168236.
  4. Yang, L, Xu, S, Guo, X, Uchida, S, Weinstein, AM, Wang, T et al.. Regulation of renal Na transporters in response to dietary K. Am. J. Physiol. Renal Physiol. 2018;315 (4):F1032-F1041. doi: 10.1152/ajprenal.00117.2018. PubMed PMID:29923764 PubMed Central PMC6230734.
  5. Frindt, G, Yang, L, Bamberg, K, Palmer, LG. Na restriction activates epithelial Na channels in rat kidney through two mechanisms and decreases distal Na+ delivery. J. Physiol. (Lond.). 2018;596 (16):3585-3602. doi: 10.1113/JP275988. PubMed PMID:29737520 PubMed Central PMC6092292.
  6. Palmer, LG. Epithelial transport in The Journal of General Physiology. J. Gen. Physiol. 2017;149 (10):897-909. doi: 10.1085/jgp.201711828. PubMed PMID:28931633 PubMed Central PMC5688356.
  7. Li, J, Hatano, R, Xu, S, Wan, L, Yang, L, Weinstein, AM et al.. Gender difference in kidney electrolyte transport. I. Role of AT1a receptor in thiazide-sensitive Na+-Cl- cotransporter activity and expression in male and female mice. Am. J. Physiol. Renal Physiol. 2017;313 (2):F505-F513. doi: 10.1152/ajprenal.00087.2017. PubMed PMID:28566500 PubMed Central PMC5582908.
  8. Frindt, G, Yang, L, Uchida, S, Weinstein, AM, Palmer, LG. Responses of distal nephron Na+ transporters to acute volume depletion and hyperkalemia. Am. J. Physiol. Renal Physiol. 2017;313 (1):F62-F73. doi: 10.1152/ajprenal.00668.2016. PubMed PMID:28356292 PubMed Central PMC5538837.
  9. Yang, L, Frindt, G, Lang, F, Kuhl, D, Vallon, V, Palmer, LG et al.. SGK1-dependent ENaC processing and trafficking in mice with high dietary K intake and elevated aldosterone. Am. J. Physiol. Renal Physiol. 2017;312 (1):F65-F76. doi: 10.1152/ajprenal.00257.2016. PubMed PMID:27413200 PubMed Central PMC5283884.
  10. Ergonul, Z, Yang, L, Palmer, LG. Properties of acid-induced currents in mouse dorsal root ganglia neurons. Physiol Rep. 2016;4 (9):. doi: 10.14814/phy2.12795. PubMed PMID:27173673 PubMed Central PMC4873640.
  11. Frindt, G, Gravotta, D, Palmer, LG. Regulation of ENaC trafficking in rat kidney. J. Gen. Physiol. 2016;147 (3):217-27. doi: 10.1085/jgp.201511533. PubMed PMID:26880754 PubMed Central PMC4772376.
  12. Palmer, LG. Piece treaties connect ENaC subunits. Channels (Austin). 2015;9 (5):223-4. doi: 10.1080/19336950.2015.1092843. PubMed PMID:26542626 PubMed Central PMC4826115.
  13. Frindt, G, Palmer, LG. Acute effects of aldosterone on the epithelial Na channel in rat kidney. Am. J. Physiol. Renal Physiol. 2015;308 (6):F572-8. doi: 10.1152/ajprenal.00585.2014. PubMed PMID:25520012 PubMed Central PMC4360037.
  14. Patel, AB, Yang, L, Deng, S, Palmer, LG. Feedback inhibition of ENaC: acute and chronic mechanisms. Channels (Austin). 2014;8 (5):444-51. doi: 10.4161/19336950.2014.949190. PubMed PMID:25483587 PubMed Central PMC4594590.
  15. Gleason, CE, Frindt, G, Cheng, CJ, Ng, M, Kidwai, A, Rashmi, P et al.. mTORC2 regulates renal tubule sodium uptake by promoting ENaC activity. J. Clin. Invest. 2015;125 (1):117-28. doi: 10.1172/JCI73935. PubMed PMID:25415435 PubMed Central PMC4382226.
  16. Carattino, MD, Mueller, GM, Palmer, LG, Frindt, G, Rued, AC, Hughey, RP et al.. Prostasin interacts with the epithelial Na+ channel and facilitates cleavage of the γ-subunit by a second protease. Am. J. Physiol. Renal Physiol. 2014;307 (9):F1080-7. doi: 10.1152/ajprenal.00157.2014. PubMed PMID:25209858 PubMed Central PMC4216988.
  17. Yang, L, Palmer, LG. Ion conduction and selectivity in acid-sensing ion channel 1. J. Gen. Physiol. 2014;144 (3):245-55. doi: 10.1085/jgp.201411220. PubMed PMID:25114023 PubMed Central PMC4144671.
  18. Palmer, LG, Schnermann, J. Integrated control of Na transport along the nephron. Clin J Am Soc Nephrol. 2015;10 (4):676-87. doi: 10.2215/CJN.12391213. PubMed PMID:25098598 PubMed Central PMC4386267.
  19. Frindt, G, Li, H, Sackin, H, Palmer, LG. Inhibition of ROMK channels by low extracellular K+ and oxidative stress. Am. J. Physiol. Renal Physiol. 2013;305 (2):F208-15. doi: 10.1152/ajprenal.00185.2013. PubMed PMID:23678039 PubMed Central PMC3725663.
  20. Patel, AB, Frindt, G, Palmer, LG. Feedback inhibition of ENaC during acute sodium loading in vivo. Am. J. Physiol. Renal Physiol. 2013;304 (2):F222-32. doi: 10.1152/ajprenal.00596.2012. PubMed PMID:23171553 PubMed Central PMC3543624.
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