Physiology, the oldest and most fundamental of the basic medical sciences, is the science of the function of living organisms, including humans. In the Department of Physiology and Biophysics, our techniques are modern and are constantly evolving, our questions are immediate, but our basic quest remains constant. As Physiologists we ask how biological processes - from molecules to organisms - perform their functions. As Biophysicists we ask how these processes - and life itself - build upon the laws of mathematics, physics, and chemistry.

Recent publications by members of the Department of Physiology and Biophysics:
  1. Doktorova M, Heberle FA, Kingston RL, Khelashvili G, Cuendet MA, Wen Y, Katsaras J, Feigenson GW, Vogt VM, Dick RA. Cholesterol Promotes Protein Binding by Affecting Membrane Electrostatics and Solvation Properties Biophys JNov 2017 PMID: 29117524
  2. Clark LD, Dikiy I, Chapman K, Rödström KE, Aramini J, LeVine MV, Khelashvili G, Rasmussen S, Gardner KH, Rosenbaum DM. Ligand modulation of sidechain dynamics in a wild-type human GPCR ElifeOct 2017 PMID: 28984574
  3. Lacko, L., Hurtado, R., Hinds, S., Poulos, M.G., Butler, J.M., Gale, N.W., Stuhlmann, H.. Altered feto-placental vascularization, placental malperfusion, and fetal growth restriction in mice with Egfl7 loss-of- function DevlopmentJul 2017 PMID: 28526753
  4. Vishwanathan A, Daie K, Ramirez AD, Lichtman JW, Aksay ERF, Seung HS. Electron Microscopic Reconstruction of Functionally Identified Cells in a Neural Integrator Curr Biol.Jul 2017 PMID: 28712570
  5. Alice Verchère, Wei-Lin Ou, Birgit Ploier, Takefumi Morizumi, Michael A. Goren, Peter Bütikofer, Oliver P. Ernst, George Khelashvili & Anant K. Menon. Light-independent phospholipid scramblase activity of bacteriorhodopsin from Halobacterium salinarum Sci Rep.Jul 2017 PMID: 28842688
  6. Weinstein AM. A mathematical model of the rat kidney: K+-induced natriuresis. Am J Physiol Renal PhysiolJun 2017 PMID: 28179254
  7. Doktorova M, Harries D, Khelashvili G. Determination of bending rigidity and tilt modulus of lipid membranes from real-space fluctuation analysis of molecular dynamics simulations Phys. Chem. Chem. Phys.Jun 2017 PMID: 28627570
  8. Madhur Srivastava, Elka R. Georgieva, Jack H. Freed.. A New Wavelet Denoising Method for Experimental Time-Domain Signals: Pulsed Dipolar Electron Spin Resonance J Phys Chem AMar 2017 PMID: 28257206
  9. Neil A. Eschmann, Elka R. Georgieva, Pritam Ganguly, Peter P. Borbat, Maxime Rappaport, Yasar Akdogan, Jack H. Freed, Joan-Emma Shea, Song-I Han. Signature of an aggregation-prone conformation of tau Chem BiolMar 2017 PMID: 28303942
  10. Razavi AM, Khelashvili G, Weinstein H. A Markov State-based Quantitative Kinetic Model of Sodium Release from the Dopamine Transporter Nat Sci RepJan 2017 PMID: 28059145
  11. Sahai MA, Davidson C, Khelashvili G, Barrese V, Dutta N, Weinstein H, Opacka-Juffry J. Combined in vitro and in silico approaches to the assessment of stimulant properties of novel psychoactive substances – The case of the benzofuran 5-MAPB J pnpbpNov 2016 PMID: 27890676
  12. Elka R. Georgieva. Nanoscale lipid membrane mimetics in spin-labeling and electron paramagnetic resonance spectroscopy studies of protein structure and function Nanotechnol RevNov 2016
  13. Cuendet MA, Weinstein H, LeVine MV. The Allostery Landscape: Quantifying Thermodynamic Couplings in Biomolecular Systems JCTCNov 2016 PMID: 27766843
  14. Khelashvili G, Schmidt SG, Shi L, Javitch JA, Gether U, Loland CJ, Weinstein H. Conformational Dynamics on the Extracellular Side of LeuT Controlled by Na+ and K+ Ions and the Protonation State of Glu290 J Biol ChemSep 2016 PMID: 27474737
  15. Elka R. Georgieva, Peter P. Borbat, Kirill Grushin, Svetla Stoilova-McPhie, Nichita J. Kulkarni, Zhichun Liang, Jack H. Freed. Conformational response of influenza A M2 transmembrane domain to amantadine drug binding at low pH (pH 5.5) Front. Physiol.Jul 2016 PMID: 27524969
  16. Veerappan A, Thompson M, Savage AR, Silverman ML, Chan WS, Sung B, Summers B, Montelione KC, Benedict P, Groh B, Vicencio AG, Peinado H, Worgall S, Silver RB. Mast cells and exosomes in hyperoxia-induced neonatal lung disease. AJPJun 2016 PMID: 27130530
  17. Hurtado*, R., and Smith, C. Hyperpolarization-activated cation and T-type calcium ion channel expression in porcine and human renal pacemaker tissues. Journal of Anatomy J Anat.May 2016 PMID: 26805464
  18. Zhao Y, Fay F, Hak S, Manuel Perez-Aguilar J, Sanchez-Gaytan BL, Goode B, Duivenvoorden R, de Lange Davies C, Bjørkøy A, Weinstein H, Fayad ZA, Pérez-Medina C, Mulder WJ. Augmenting drug-carrier compatibility improves tumour nanotherapy efficacy. Nat CommunApr 2016 PMID: 27071376
  19. Johner N, Harries D, Khelashvili G. Implementation of a methodology for determining elastic properties of lipid assemblies from molecular dynamics simulations BMC BioinfApr 2016 PMID: 27071656
  20. LeVine MV, Cuendet MA, Khelashvili G, Weinstein H. Allosteric Mechanisms of Molecular Machines at the Membrane: Transport by Sodium-Coupled Symporters Chem RevFeb 2016 PMID: 26892914
  21. LeVine MV, Khelashvili G, Shi L, Quick M, Javitch JA, Weinstein H. Role of Annular Lipids in the Functional Properties of Leucine Transporter LeuT Proteomicelles BiochemFeb 2016 PMID: 26811944
  22. Arango-Lievano M, Sensoy O, Borie A, Corbani M, Guillon G, Sokoloff P, Weinstein H, Jeanneteau F. A GIPC1-Palmitate Switch Modulates Dopamine Drd3 Receptor Trafficking and Signaling Mol Cell BiolJan 2016 PMID: 26787837
  23. Stolzenberg S, Michino M, LeVine MV, Weinstein H, Shi L. Computational approaches to detect allosteric pathways in transmembrane molecular machines Biochim Biophys Acta.Jan 2016 PMID: 26806157
  24. Benjamin Orlando, Peter P. Borbat, Elka R. Georgieva, Jack H. Freed, Michael G. Malkowski. Pulsed dipolar spectroscopy reveals that tyrosyl radicals are generated in both monomers of the cyclooxygenase-2 dimer BiochemDec 2015 PMID: 26636181
  25. Khelashvili G, Stanley N, Sahai MA, Medina J, LeVine MV, Shi L, De Fabritiis G, Weinstein H. Spontaneous Inward Opening of the Dopamine Transporter Is Triggered by PIP2‐Regulated Dynamics of the N‐Terminus ACS Chem NeurosciNov 2015 PMID: 26255829
  26. Billesbølle CB, Krüger MB, Shi L, Quick M, Li Z, Stolzenberg S, Kniazeff J, Gotfryd K, Mortensen JS, Javitch JA, Weinstein H, Loland CJ, Gether U. Substrate-induced Unlocking of the Inner Gate Determines the Catalytic Efficiency of a Neurotransmitter:Sodium Symporter J Biol ChemOct 2015 PMID: 26363074
  27. Khelashvili G, Weinstein H. Functional mechanisms of neurotransmitter transporters regulated by lipid-protein interactions of their terminal loops Biochim Biophys Acta.Sep 2015 PMID: 25847498
  28. Hurtado, R., Zewdu, R., Mtui, J., Liang, C., Aho, R., Kurylo, C., Selleri, L., Herzlinger, D.. Pbx1-dependent control of VMC differentiation kinetics underlies gross renal vascular patterning. Development DevlopmentAug 2015 PMID: 26138478
  29. Elka R. Georgieva, Peter P. Borbat, Haley D. Norman and Jack H. Freed. Mechanism of influenza A M2 transmembrane domain assembly in lipid membranes Sci Rep.Jul 2015 PMID: PMC4507135
  30. Karlsen ML, Thorsen TS, Johner N, Ammendrup-Johnsen I, Erlendsson S, Tian X, Simonsen JB, Høiberg-Nielsen R, Christensen NM, Khelashvili G, Streicher W, Teilum K, Vestergaard B, Weinstein H, Gether U, Arleth L, Madsen KL. Structure of Dimeric and Tetrameric Complexes of the BAR Domain Protein PICK1 Determined by Small-Angle X-Ray Scattering StructureJul 2015 PMID: 26073603
  31. Weinstein AM. A mathematical model of rat proximal tubule and loop of Henle Am J Physiol Renal PhysiolMay 2015 PMID: 25694479
  32. Weinstein AM. A mathematical model of the rat nephron: glucose transport. Am J Physiol Renal PhysiolMay 2015 PMID: 25694480
  33. Lee MM, Arrenberg AB, Aksay ER. A structural and genotypic scaffold underlying temporal integration J NeurosciMay 2015 PMID: 25995475
  34. LeVine MV, Weinstein H. AIM for Allostery: Using the Ising Model to Understand Information Processing and Transmission in Allosteric Biomolecular Systems EntropyMay 2015 PMID: 26594108
  35. Khelashvili G, Doktorova M, Sahai MA, Johner N, Shi L, Weinstein H. Computational modeling of the N-terminus of the human dopamine transporter and its interaction with PIP2-containing membranes ProteinsMay 2015 PMID: 25739722
  36. Stolzenberg S, Quick M, Zhao C, Gotfryd K, Khelashvili G, Gether U, Loland CJ, Javitch JA, Noskov S, Weinstein H, Shi L. Mechanism of the Association between Na+ Binding and Conformations at the Intracellular Gate in Neurotransmitter:Sodium Symporters J Biol ChemMay 2015 PMID: 25869126
  37. Sensoy O, Weinstein H. A mechanistic role of Helix 8 in GPCRs: Computational modeling of the dopamine D2 receptor interaction with the GIPC1–PDZ-domain Biochim Biophys Acta.Apr 2015 PMID: 25592838
  38. Kulig W, Olżyńska A, Jurkiewicz P, Kantola AM, Komulainen S, Manna M, Pourmousa M, Vazdar M, Cwiklik L, Rog T, Khelashvili G, Harries D, Telkki VV, Hof M, Vattulainen I, Jungwirth P. Cholesterol under oxidative stress—How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols Free Radic Biol Med. Mar 2015 PMID: 25795515
  39. Daie K, Goldman MS, Aksay ERF. Spatial patterns of persistent neural activity vary with the behavioral context of short-term memory NeuronFeb 2015 PMID: 25661184
  40. Akyuz N, Georgieva ER, Zhou Z, Stolzenberg S, Cuendet MA, Khelashvili G, Altman RB, Terry DS, Freed JH, Weinstein H, Boudker O, Blanchard SC. Transport domain unlocking sets the uptake rate of an aspartate transporter NatureFeb 2015 PMID: 25652997
  41. Herzlinger, D., Hurtado, R.. Patterning of the Renal vascular bed Semin Cell Dev BiolDec 2014 PMID: 25128732
  42. Johner N, Harries D, Khelashvili G. Curvature and Lipid Packing Modulate the Elastic Properties of Lipid Assemblies: Comparing HII and Lamellar Phases J. Phys. Chem. Lett.Nov 2014 PMID: 26278954
  43. Perez-Aguilar JM, Shan J, LeVine MV, Khelashvili G, Weinstein H. A Functional Selectivity Mechanism at the Serotonin-2A GPCR Involves Ligand-Dependent Conformations of Intracellular Loop 2 J Am Chem SocNov 2014 PMID: 25314362
  44. Handschuh K, Feenstra J, Koss M, Ferretti E, Risolino M, Zewdu R, Sahai MA, Bénazet JD, Peng XP, Depew MJ, Quintana L, Sharpe J, Wang B, Alcorn H, Rivi R, Butcher S, Manak JR, Vaccari T, Weinstein H, Anderson KV, Lacy E, Selleri L. ESCRT-II/Vps25 constrains digit number by endosome-mediated selective modulation of FGF-SHH signaling. Cell RepOct 2014 PMID: 25373905
  45. Elka R. Georgieva, Shifeng Xiao, Peter P. Borbat, Jack H. Freed and David Eliezer. Tau binds to membrane surfaces via short amphipathic helices located in its microtubule-binding repeats Biophys JSep 2014 PMID: 25229151
  46. Borre L, Andreassen TF, Shi L, Weinstein H, Gether U. The second sodium site in the dopamine transporter controls cation permeation and is regulated by chloride J Biol ChemSep 2014 PMID: 25063810
  47. Lacko, L. A., Massimiani, M., Sones, J. L., Hurtado, R., Salvi, S., Ferrazzani, S., Davisson, R. L., Campagnolo, L., Stuhlmann, H.. Novel expression of EGFL7 in placental trophoblast and endothelial cells and its implication in preeclampsia Mech Dev.Aug 2014 PMID: 24751645
  48. Dehnes Y, Shan J, Beuming T, Shi L, Weinstein H, Javitch J. Conformational changes in dopamine transporter intracellular regions upon cocaine binding and dopamine translocation. Neurochemistry InternationalJul 2014 PMID: 24576496
  49. Hansen FH, Skjørringe T, Yasmeen S, Arends NV, Sahai MA, Erreger K, Andreassen TF, Holy M, Hamilton PJ, Neergheen V, Karlsborg M, Newman AH, Pope S, Heales SJ, Friberg L, Law I, Pinborg LH, Sitte HH, Loland C, Shi L, Weinstein H, Galli A, Hjermind LE, Møller LB, Gether U. Missense dopamine transporter mutations associate with adult parkinsonism and ADHD J Clin InvestJul 2014 PMID: 24911152
  50. Mondal S, Khelashvili G, Weinstein H. Not Just an Oil Slick: How the Energetics of Protein-Membrane Interactions Impacts the Function and Organization of Transmembrane Proteins Biophys JJun 2014 PMID: 24896109
  51. Hamilton PJ, Belovich AN, Khelashvili G, Saunders C, Erreger K, Javitch JA, Sitte HH, Weinstein H, Matthies HJ, Galli A. PIP2 regulates psychostimulant behaviors through its interaction with a membrane protein Nat Chem BiolJun 2014 PMID: 24880859
  52. Kazmier K, Sharma S, Quick M, Islam SM, Roux B, Weinstein H, Javitch J, McHaourab H. Conformational dynamics of ligand-dependent alternating access in LeuT Nat Struct Mol BiolMay 2014 PMID: 24747939
  53. Verdon G, Oh S, Serio RN, Boudker O. Coupled ion binding and structural transitions along the transport cycle of glutamate transporters ElifeMay 2014 PMID: 24842876
  54. LeVine M, Weinstein H. NbIT - A New Information Theory-Based Analysis of Allosteric Mechanisms Reveals Residues that Underlie Function in the Leucine Transporter LeuT PLoS Comp BiolMay 2014 PMID: 24785005
  55. LeVine M,Perez-Aguilar JM,Weinstein H. N-body Information Theory (NbIT) Analysis of Rigid- Body Dynamics in Intracellular Loop 2 of the 5-HT2A Receptor Proceedings IWBBIO Apr 2014
  56. Hurtado, R., Bub, G., and Herzlinger, D.. A molecular signature of tissues with pacemaker activity in the heart and upper urinary tract involves coexpressed hyperpolarization-activated cation and T-type Ca2+ channels FASEB J.Feb 2014 PMID: 24189942
  57. Johner N, Mondal S, Morra G, Caffrey M,Weinstein H, and Khelashvil G. Protein and Lipid Interactions Driving Molecular Mechanisms of in meso Crystallization. J Am Chem SocFeb 2014 PMID: 24494670
  58. Khelashvili G, Johner N, Zhao G, Harries D, Scott H.L. Molecular origins of bending rigidity in lipids with isolated and conjugated double bonds: The effect of cholesterol Chem Phys LipJan 2014 PMID: 24394210
  59. Mondal S, Khelashvili G, Johner N, and Weinstein H. How the Dynamic Properties and Functional Mechanisms of GPCRs Are Modulated by Their Coupling to the Membrane Environment Adv Exp Med BiolJan 2014 PMID: 24158801