Research in our laboratory is geared toward understanding how ion channel protein structure and mechanism interrelate at the molecular level to allow channels to elaborate various biological properties. We use a combination of molecular, biochemical and electrophysiological approaches to evaluate in a complete fashion fundamental channel properties within the context of emerging structural data.
Please see our Research and People pages for more details.
Schmidpeter PA, Gao X, Uphadyay V, Rheinberger J, Nimigean CM. Ligand binding and activation properties of the purified bacterial cyclic nucleotide-gated channel SthK. J Gen Physiol. 2018 May 11. pii: jgp.201812023
Kowal J, Biyani N, Chami M, Scherer S, Rzepiela AJ, Baumgartner P, Upadhyay V, Nimigean CM, Stahlberg H. High-Resolution Cryoelectron Microscopy Structure of the Cyclic Nucleotide-Modulated Potassium Channel MloK1 in a Lipid Bilayer. Structure. 2018 Jan 2;26(1):20-27.e3.
Posson DJ, Rusinova R, Andersen OS, Nimigean CM. Stopped-Flow Fluorometric Ion Flux Assay for Ligand-Gated Ion Channel Studies. Methods Mol Biol. 2018;1684:223-235.
Posson DJ, Rusinova R, Andersen OS, Nimigean CM. (2015). Calcium ions open a selectivity filter gate during activation of the MthK potassium channel. Nat Commun. 2015 Sep 23;6:8342.
Kowal J, Chami M, Baumgartner P, Arheit M, Chiu PL, Rangl M, Scheuring S, Schröder GF, Nimigean CM, Stahlberg H. (2014). Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nat Commun. 2014;5:3106. doi: 10.1038/ncomms4106.
Posson DJ, McCoy JG, Nimigean CM. (2013). The voltage-dependent gate in MthK potassium channels is located at the selectivity filter. Nat Struct Mol Biol. 2013 Feb;20(2):159-66.
Nimigean C.M., Allen T.W. (2011). Origins of ion selectivity in potassium channels from the perspective of channel block. Journal of General Physiology, 137(5):405-13.
Banerjee S and Nimigean C.M. (2011). Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers. Journal of General Physiology, 137(2):217-23 (Cover article)
Thompson A.N., Kim I, Panosian T.D., Iverson T.M., Allen T.W., and Nimigean C.M. (2009). Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat. Struc. Mol. Biol., 16(12):1317-24.
Thompson A.N., Posson D.J., Parsa P.V., and Nimigean C.M. (2008). Molecular mechanism of pH sensing in KcsA potassium channels. PNAS, 105(19):6900-5. Epub 2008 Apr 28
Chiu P.-L., Pagel M., Evans J., Chou H.-T., Zeng X., Gipson B., Stahlberg H., and Nimigean C. M. (2007). The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16Å resolution. Structure, 15(9):1053-64 (Accompanied by preview)
Crina M. Nimigean (2006). A radioactive uptake assay for the measurement of ion transport across ion channel-containing liposomes. Nature Protocols, 1.3:1207-1212. Invited protocol.
Nimigean, C.M., Shane T., and Miller, C (2004). A cyclic nucleotide modulated prokaryotic K+ channel. Journal of General Physiology, 124:203-210 (Accompanied by commentary)
Nimigean, C.M., Chappie, J.S. and Miller, C (2003). Electrostatic tuning of ion conductance in potassium channels. Biochemistry, 42:9263-9268
Nimigean, C.M. and Magleby, K.L. (1999). The beta subunit increases the Ca2+ sensitivity of large conductance Ca2+-activated potassium channels by retaining the gating in the bursting states. Journal of General Physiology, 113:425-439