Research in our laboratory is geared toward understanding how ion channel protein structure and mechanism interrelate at the molecular level to allow channels to elaborate various biological properties. We use a combination of molecular, biochemical and electrophysiological approaches to evaluate in a complete fashion fundamental channel properties within the context of emerging structural data.

Please see our Research and People pages for more details.



Rusinova R., Kim D., Nimigean C.M., Andersen O.S. (2014). Regulation of ion channel function by the host lipid bilayer examined by a stopped-flow spectrofluorimetric assay. Biophysical Journal, 106 (5), doi: 10.1016/j.bpj.2014.01.027.

McCoy J.G., Rusinova R., Kim D., Kowal J., Banerjee S., Alexis Jaramillo Cartagena, Thompson A.N., Kolmakova-Partensky L., Stahlberg H., Andersen O.S., Nimigean C.M. (2014). A KcsA/MloK1 chimeric ion channel has lipid-dependent ligand binding energetics. Journal of Biological Chemistry, doi:10.1074/jbc.M113.543389.

Kowal J., Chami M., Baumgartner P., Arheit M., Chiu P., Rangl M., Scheuring S., Schroder G.F., Nimigean C.M., Stahlberg H. (2014). Ligand-induced structural changes in the cyclic nucleotide-modulated potassium channel MloK1. Nature communications, 5: 3106, doi: 10.1038/ncomms4106.

Posson D.P., Thompson A.N., McCoy J.G., Nimigean C.M. (2013). Molecular interactions involved in proton-dependent gating in KcsA potassium channels. Journal of General Physiology, 142 (6):613-624.

Bhate M.P., Wylie B.J., Thompson A., Tian L., Nimigean C.M., and McDermott A.E. (2013). Preparation of uniformly isotope labeled KcsA for solid state NMR: Expression, purification, reconstitution into liposomes and functional assay. Protein Expression and Purification, ISSN 1046-5928, doi:10.1016/j.pep.2013.07.013.

Posson D.J., McCoy J.G., and Nimigean C.M. (2013). The voltage-dependent gate in MthK potassium channels is located at the selectivity filter. Nature Structural and Molecular Biology, doi:10.1038/nsmb.2473

McCoy J.G., Nimigean C.M. (2012). Structural correlates of selectivity and inactivation in potassium channels. Biochim Biophys Acta. 1818(2):272-85.

Nimigean C.M., Allen T.W. (2011). Origins of ion selectivity in potassium channels from the perspective of channel block. Journal of General Physiology, 137(5):405-13.

Cheng W.W., McCoy J.G., Thompson A.N., Nichols C.G., Nimigean C.M. (2011). Mechanism for selectivity-inactivation coupling in KcsA potassium channels. PNAS, 108(13):5272-7.

Banerjee S and Nimigean C.M. (2011). Non-vesicular transfer of membrane proteins from nanoparticles to lipid bilayers. Journal of General Physiology, 137(2):217-23 (Cover article)

Tanabe M, Nimigean C.M., Iverson T.M. (2010). Structural basis for solute transport, nucleotide regulation, and immunological recognition of Neisseria meningitidis PorB. PNAS, 107(15):6811-6.

Thompson A.N., Kim I, Panosian T.D., Iverson T.M., Allen T.W., and Nimigean C.M. (2009). Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Nat. Struc. Mol. Biol., 16(12):1317-24. Epub 2009 Nov 29

Thompson A.N., Posson D.J., Parsa P.V., and Nimigean C.M. (2008). Molecular mechanism of pH sensing in KcsA potassium channels. PNAS, 105(19):6900-5. Epub 2008 Apr 28

Chiu P.-L., Pagel M., Evans J., Chou H.-T., Zeng X., Gipson B., Stahlberg H., and Nimigean C. M. (2007). The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16Å resolution. Structure, 15(9):1053-64 (Accompanied by preview)

Nimigean C.M. and Pagel M.D. (2007). Ligand binding and activation in a prokaryotic cyclic nucleotide-modulated channel. Journal of Molecular Biology, 371(5):1325-37. Epub 2007 Jun 15.

Crina M. Nimigean
(2006). A radioactive uptake assay for the measurement of ion transport across ion channel-containing liposomes. Nature Protocols, 1.3:1207-1212. Invited protocol.

Zadek B. and Nimigean C.M. (2006). Calcium dependent gating of MthK, a prokaryotic potassium channel. Journal of General Physiology, 127:673-685

Nimigean, C.M., Shane T., and Miller, C (2004). A cyclic nucleotide modulated prokaryotic K+ channel. Journal of General Physiology, 124:203-210 (Accompanied by commentary)

Nimigean, C.M
., Chappie, J.S. and Miller, C (2003). Electrostatic tuning of ion conductance in potassium channels. Biochemistry, 42:9263-9268

Qian X, Nimigean CM, Niu X, Moss BL, Magleby KL (2002). Slo1 tail domains, but not the Ca2+ bowl, are required for the beta1 subunit to increase the apparent Ca2+ sensitivity of BK channels. Journal of General Physiology, 120:829-43.

Nimigean, C.M. and Miller, C. (2002). Na+ block and permeation in a K+ channel of known structure. Journal of General Physiology, 120:323-335

Nimigean, C.M. and Magleby, K.L. (2000). Functional coupling of the b1 subunit to the large conductance Ca2+-activated K+ channel in the absence of Ca2+. Increased Ca2+ sensitivity from a Ca2+-independent mechanism. Journal of General Physiology, 115:719-734

Moss, B.L., Silberberg, S.D., Nimigean, C.M. and Magleby, K.L. (1999). Ca2+-dependent gating mechanisms for dslo, a large conductance Ca2+-activated K+ (BK) channel. Biophysical Journal, 76:3099-3117

Nimigean, C.M. and Magleby, K.L. (1999). The b subunit increases the Ca2+-sensitivity of large conductance Ca2+-activated potassium channels by retaining the gating in the bursting states. Journal of General Physiology, 113:425-439

Brett, C.M.A. and Nimigean, C.M. (1997). Corrosion of sputtered W--Ni--N hard coatings in chloride media. Thin Solid Films, 311 (1-2):1-6

Crina Nimigean, Ph.D.
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